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KMID : 1149220170250040483
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Korean Journal of Oriental Medical Prescription
2017 Volume.25 No. 4 p.483 ~ p.497
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Neuro-inflammation induced by restraint stress causes impairs neurobehavior in mice
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Oh Tae-Woo
Do Hyun-Ju
Kim Kwang-Youn
Kim Young-Woo
Lee Byung-Wook
Ma Jin-Yeul
Park Kwang-Il
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Abstract
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Background : Behavioral stress has been suggested as one of the significant factors that is able to disrupt physiological systems and cause depression as well as changes in various body systems. The stressful events can alter cognition, learning, memory and emotional responses, resulting in mental disorders such as depression and anxiety.
Results : We used a restraint stress model to evaluate the alteration of behavior and stress-related blood parameter. The animals were randomly divided into two groups of five animals each group. Furthermore, we assessed the change of body weight to evaluate the locomotor activity as well as status of emotional and anxiety in mice. After 7 days of restraint stress, the body weight had significantly decreased in the restraint stress group compared with the control group. We also observed stress-associated behavioral alterations, as there was a significant decrease in open field and forced swim test, whereas the immobilization time was significantly increased in the stress group compared to the control group. We observed the morphological changes of neuronal death and microglia by immunohistochemistry and western blot. In our study restraint stress did not cause change in neuronal cell density in the frontal cortex and CA1 hippocampus region, but there was a trend for an increased COX-2 and iNOS protein expression and microglia (CD11b) in brain, which is restraint stress.
Conclusion : Our study, there were significant alterations observed in the behavioral studies. We found that mice undergoing restraint stress changed behavior, confirming the increased expression of inflammatory factors in the brain.
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KEYWORD
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restraint stress , anxiety , depression , neuro-inflammatory , neurobehavior
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